Table 1.
Identification of TNF-α protein levels in the sera of MHV infected mice by ELISA
| Mouse strain | Day P.I. | MHV-injected |
Mock-infected |
||
|---|---|---|---|---|---|
| Pos/totala | (pg/ml)b | Pos/totala | (pg/ml)b | ||
| BALB/c | 4 | 9/9 | 56 ± 15c | 0/8 | 0 |
| 8 | 0/7 | 0 | 0/7 | 0 | |
| 20 | 0/11 | 0 | 0/5 | 0 | |
| CD-1 | 4 | 2/8 | 22 ± 20 | 0/7 | 0 |
| 8 | 3/7 | 35 ± 20 | 1/7 | 11 ± 11 | |
| 20 | 1/9 | 1 ± 1 | 0/7 | 0 | |
The sensitivity of the ELISA is 5 pg/ml thus values above this are considered to be positive.
a
The number of animals where TNF-α was detected over the number tested.
b
Average TNF-α levels ± the standard error of the mean.
c
p < 0.005 compared to mock-infected controls.