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. 2009 May 4;160(1):111–118. doi: 10.1016/j.jviromet.2009.04.029

Fig. 1.

Fig. 1

Analytical and diagnostic specificity of reverse transcription multiplex real-time PCR. The HEX, Cy5, and FAM fluorescence were genotype 1 (black real line), 2 (green broken line), and 3 (red broken line), respectively. The x-axis represents cycle number. The y-axis is the amount of fluorescent signal detected. Sample 1: standard plasmid of genotypes 1, 2, and 3; samples 2–17: non-CSFV swine virus; samples 18–43: buffy coats and tissue emulsions from an SPF farm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of the article.)