Table 3.

Reproducibility assay of threshold cycle value quantified by RT-MRT-PCR from the standard plasmid.

Plasmid	Concentrationa (viral copy/μl)	Intra-assay			Inter-assay
		Mean	S.D.	CV (%)	Mean	S.D.	CV (%)
Genotype 1	101	39.25	0.60	1.54	39.12	0.17	0.44
	104	27.56	0.13	0.46	27.41	0.26	0.93
	107	17.82	0.18	1.00	18.13	0.29	1.60
Genotype 2	101	38.25	0.31	0.82	38.33	0.45	1.17
	104	27.02	0.15	0.56	26.98	0.11	0.40
	107	17.41	0.36	2.08	17.96	0.32	1.76
Genotype 3	101	38.33	0.61	1.60	37.50	0.37	0.99
	104	26.69	0.05	0.19	26.62	0.08	0.31
	107	16.86	0.37	2.17	17.49	0.26	1.48

^aEach concentration of standard plasmid contained plasmids of genotypes 1, 2, and 3 and simultaneously detected three genotypes by RT-MRT-PCR. Each standard plasmid was detected in triplicate by RT-MRT-PCR in the intra-assay and inter-assay.