Table 4.
Summary of the sensitivity of viral isolation, RT-PCR, RT-nPCR, and RT-MRT-PCR.
| CSFV straina (genotype) | Dilution | Viral isolation | RT-PCR | RT-nPCR | RT-MRT-PCR |
|---|---|---|---|---|---|
| C-strain (1.1) | 10−1 | 8/8 | + | + | + |
| 10−2 | 8/8 | + | + | + | |
| 10−3 | 8/8 | + | + | + | |
| 10−4 | 8/8 | + | + | + | |
| 10−5 | 8/8 | − | + | + | |
| 10−6 | 4/8 | − | + | + | |
| 10−7 | 1/8 | − | − | − | |
| 10−8 | 0/8 | − | − | − | |
| TCID50: 106/ml | |||||
| Q90-278 (2.1) | 10−1 | 8/8 | + | + | + |
| 10−2 | 8/8 | + | + | + | |
| 10−3 | 8/8 | − | + | + | |
| 10−4 | 0/8 | − | − | − | |
| 10−5 | 0/8 | − | − | − | |
| 10−6 | 0/8 | − | − | − | |
| 10−7 | 0/8 | − | − | − | |
| 10−8 | 0/8 | − | − | − | |
| TCID50: 103.5/ml | |||||
| 83-19 (3.4) | 10−1 | 8/8 | + | + | + |
| 10−2 | 8/8 | + | + | + | |
| 10−3 | 8/8 | + | + | + | |
| 10−4 | 7/8 | + | + | + | |
| 10−5 | 4/8 | − | + | + | |
| 10−6 | 0/8 | − | − | − | |
| 10−7 | 0/8 | − | − | − | |
| 10−8 | 0/8 | − | − | − | |
| TCID50: 105/ml | |||||
a
CSFV strains were 10-fold serially diluted and CSFV was detected from viral isolation, RT-PCR, RT-nPCR, and RT-MRT-PCR. The TCID50 of CSFV strains was calculated from result of viral isolation.