. 2009 May 4;160(1):111–118. doi: 10.1016/j.jviromet.2009.04.029

Table 5.

The agreement among viral isolation, RT-PCR, RT-nPCR, and RT-MRT-PCR^a.

Method	Result	Viral isolation
		Positive	Negative	Kappa values	Sensitivity (%)	Specificity (%)
RT-MRT-PCR	Positive	101	11	93.5	100	83.8
RT-MRT-PCR	Negative	0	57	93.5	100	83.8

RT-PCR	Positive	101	7	95.9	100	89.7
RT-PCR	Negative	0	61	95.9	100	89.7

RT-nPCR	Positive	101	10	94.1	100	85.3
RT-nPCR	Negative	0	58	94.1	100	85.3

A total of 169 clinical samples was detected for the presence of CSFV and the results included and used to evaluate agreement among viral isolation, RT-PCR, RT-nPCR, and RT-MRT-PCR. Agreement among tests was based on kappa statistics (Thrusfield, 1995).