Table 6.
Detection of wild-type CSFV in plasma of vaccinated exposed pigs by RT-MRT-PCRa.
| ID of pigs | Group | 0 DPC | 2 DPC | 4 DPC | 6 DPC | 8 DPC | 10 DPC | 12 DPC | 14 DPC | 21 DPC |
|---|---|---|---|---|---|---|---|---|---|---|
| 1212 | C-strain | −b | − | − | − | 3.34 | − | − | − | − |
| 1214 | C-strain | − | − | − | − | 3.77 | − | − | − | − |
| 1416 | C-strain | − | − | − | − | − | − | − | − | − |
| 1217 | C-strain | − | − | − | − | − | − | − | − | − |
| 1218 | E2 subunit | − | − | − | 5.49 | − | − | − | − | − |
| 1219 | E2 subunit | − | − | − | − | 4.49 | 4.79 | 3.36 | − | − |
| 1220 | E2 subunit | − | − | − | − | 5.8 | 3.35 | − | − | − |
| 1221 | E2 subunit | − | − | − | 3.36 | 3.88 | − | − | − | − |
| 1222 | E2 subunit | − | − | − | − | − | − | − | − | − |
| 1213 | Control | − | 3.4 | 6.3 | 7.3 | 10 | npc | np | np | np |
| 1215 | Control | − | 3.31 | 4.34 | 7.52 | 8.21 | 4.99 | 4.78 | ( | ( |
−, negative; np, not performed.
a
The pigs in the C-strain group were vaccinated once at 8 weeks of age with one dose of attenuated lapinized C-strain vaccine of CSFV. The pigs in the E2 subunit group were vaccinated twice with one dose of E2 subunit vaccine, at 6 and 10 weeks of age. The control pigs were not vaccinated. At 12 weeks, all pigs were placed in the same room with the wild-type CSFV-infected pig. Plasma samples were collected from all pigs at 0, 2, 4, 6, 8, 10, 12, 14, and 21 days post-contact (DPC).
b
The unit of concentration of CSFV in plasma determined by RT-MRT-PCR was log viral copy/ml.
c
The pig 1213 was death at 7 DPC.