Table 1.

Sequences of template deoxynucleotides for siRNAs used for target genes

Gene targets	Sequences
T7 promoter	5′-TAATACGACTCACTATAGGAGACAGG-3′
EGFP-siRNA	Antisense: 5′-AAGCTGACCCTGAAGTTCATCCCTGTCTC-3′
	Sense: 5′-AAGATGAACTTCAGGGTCAGCCCTGTCTC-3′
Scramble siRNA	Antisense: 5′-AACAAGTCTCGTATGTAGTGGCCTGTCTC-3′
	Sense: 5′-AACCACTACATACGAGACTTGCCTGTCTC-3′
M-siRNA1	Antisense: 5′-AACGGTACTATTACCGTTGAGCCTGTCTC-3′
	Sense: 5′-AACTCAACGGTAATAGTACCGCCTGTCTC-3′
M-siRNA2	Antisense: 5′-AACTCCTGGAACAATGGAACCCCTGTCTC-3′
	Sense: 5′-AAGGTTCCATTGTTCCAGGAGCCTGTCTC-3′
M-siRNA3	Antisense: 5′-AACGACAATATTGCTTTGCTACCTGTCTC-3′
	Sense: 5′-AATAGCAAAGCAATATTGTCGCCTGTCTC-3′

Note: The eight nucleotides (nt) underlined sequences of template deoxynucleotides for each siRNA are complemented with those of T7 promoter primer. M-siRNA1, M-siRNA2 and M-siRNA3 target to nt 10–28, nt 41–59 and nt 637–655 of SCoV membrane gene, respectively.