Table 2.
Non-retention of HCoV 229E by the “in-house” Sephadex™ columns. Results, expressed as log10 of 50% cell culture infective dose (CCID50), represent the average of viral titers of at least 3 independent experiments. These assays allowed to evaluate the non-retention of the human coronavirus 229E (HCoV 229E), for each Sephadex™ type and each contact time tested. Viral suspensions were diluted at 1:10 in sterile distilled water for the specified contact time before filtration on the “in-house” Sephadex™ columns and inoculation to the L-132 cells. nd: not determined.
| Contact time (min) | Sephadex™ G-10 |
Sephadex™ G-25 |
||||
|---|---|---|---|---|---|---|
| Without filtration | After filtration | Difference | Without filtration | After filtration | Difference | |
| 0 | 5.8 ± 0.5 (n = 3) | 5.7 ± 0.6 (n = 3) | 0.1 ± 0.1 (n = 3) | 6.0 ± 0.6 (n = 6) | 5.8 ± 0.6 (n = 6) | 0.2 ± 0.2 (n = 6) |
| 5 | nd | nd | nd | 5.9 ± 0.7 (n = 6) | 5.6 ± 0.6 (n = 6) | 0.4 ± 0.2 (n = 6) |
| 15 | nd | nd | nd | 5.6 ± 0.5 (n = 6) | 5.6 ± 0.4 (n = 6) | ± 0.3 (n = 6) |
| 30 | 6.7 ± 0.9 (n = 3) | 6.4 ± 0.3 (n = 3) | 0.3 ± 0.4 (n = 3) | 6.1 ± 0.2 (n = 6) | 6.0 ± 0.4 (n = 6) | 0.1 ± 0.2 (n = 6) |
| 60 | 6.7 ± 0.9 (n = 3) | 6.4 ± 0.7 (n = 3) | 0.2 ± 0.2 (n = 3) | 6.1 ± 0.2 (n = 6) | 6.1 ± 0.1 (n = 6) | 0.0 ± 0.2 (n = 6) |