Table 3.

Elimination of potential cytotoxicity by filtration on the “in-house” Sephadex™ columns. 50% inhibitory concentration (IC₅₀) and 50% cytotoxic concentration (CC₅₀) of chlorhexidine (CHX) and hexamidine (HXM), without filtration, on L-132 cells were evaluated with methyl thiazol tetrazolium (MTT) assays and neutral red (NR) assays, respectively (in bold). IC₅₀ and CC₅₀ of (i) CHX solutions at 10⁻³ mol L⁻¹ and 10⁻⁴ mol L⁻¹, and (ii) of HXM solutions at 10⁻² mol L⁻¹ and 10⁻³ mol L⁻¹, after filtration on the “in-house” Sephadex™ G-25 and Sephadex™ G-10 columns, respectively, were then evaluated. Results, expressed in mol L⁻¹, represent the average of 3 independent experiments.

	Ci	24 h	48 h	168 h
CHX
IC50	Without filtration	(4.3 ± 0.6) × 10−6	(2.4 ± 1.3) × 10−6	(3.9 ± 0.2) × 10−6
	10−3 mol L−1	>10−4	>10−4	>5.6 × 10−5
	10−4 mol L−1	>10−5	>10−5	>10−5
CC50	Without filtration	(5.6 ± 1.1) × 10−6	(2.9 ± 1.3) × 10−6	(3.4 ± 0.5) × 10−6
	10−3 mol L−1	>10−4	>10−4	>5.4 × 10−5
	10−4 mol L−1	>10−5	>10−5	>10−5
HXM
IC50	Without filtration	(3.8 ± 1.0) × 10−5	(1.2 ± 1.4) × 10−5	(3.8 ± 2.5) × 10−6
	10−2 mol L−1	(5.3 ± 1.4) × 10−5	(1.0 ± 0.5) × 10−5	(5.9 ± 1.4) × 10−6
	10−3 mol L−1	>10−4	>10−4	>10−4
CC50	Without filtration	(6.2 ± 1.4) × 10−5	(2.5 ± 1.5) × 10−5	(5.6 ± 0.1) × 10−6
	10−2 mol L−1	(6.2 ± 1.3) × 10−5	(3.2 ± 2.2) × 10−5	(5.7 ± 0.3) × 10−6
	10−3 mol L−1	>10−4	>10−4	>5.5 × 10−5