Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Drosophila melanogaster) | Staufen (Stau) | DOI: 10.1083/jcb.201709174 | FBgn0003520; CG5753 | |
| Gene (Drosophila melanogaster) | kinesin heavy chain (khc) | Isabel Palacios lab; DOI: 10.1242/dev.097592 | FBgn0001308; CG7765 |
|
| Gene (Drosophila melanogaster) | myosin-V (didum) | DOI: 10.1074/jbc.M505209200 | FBgn0261397; CG2146 | |
| Gene (Drosophila melanogaster) | oskar (osk) | DOI: 10.1083/jcb.201709174 | FBgn0003015; CG10901 | |
| Gene (Drosophila melanogaster) | Klp10A | DGRC cDNA clone, LD29208 | FBgn0030268; CG1453 | |
| Gene (Drosophila melanogaster) | Patronin | Uri Abdu lab (Ben Gurion University) | FBgn0263197; CG33130 | |
| Genetic reagent (Drosophila melanogaster) | khcΔhinge2 | Jill Wildonger lab (University of Wisconsin-Madison); DOI: 10.1083/jcb.201708096 | ||
| Genetic reagent (Drosophila melanogaster) | Khc-RNAi (GL00330, Valium22, attP2) | Bloomington stock center | BDSC: #35409 (GL00330) | |
| Genetic reagent (Drosophila melanogaster) | maternal α-tubulin67C-Gal4-VP16[V2H] | Bloomington stock center | BDSC: #7062 | |
| Genetic reagent (Drosophila melanogaster) | maternal α-tubulin67C-Gal4-VP16[V37] | Bloomington stock center | BDSC: #7063 | |
| Genetic reagent (Drosophila melanogaster) | nos-Gal4-VP16 (III) | Edwin Ferguson lab (University of Chicago); DOI: 10.1016/s0960-9822(98)70091-0 | ||
| Genetic reagent (Drosophila melanogaster) | hs-Flp[12]; Sco/CyO | Bloomington stock center | BDSC: #1929 | |
| Genetic reagent (Drosophila melanogaster) | FRTG13, Ubi-GFP.nls.2R1, Ubi-GFP.2R2 | Bloomington stock center | BDSC: #5826 | |
| Genetic reagent (Drosophila melanogaster) | FRTG13 | Bloomington stock center | BDSC: #1956 | |
| Genetic reagent (Drosophila melanogaster) | UASp-MyoV.FL-GFP (III) | Anne Ephrussi lab (EMBL); DOI: 10.1016/j.cub.2009.04.062 | ||
| Genetic reagent (Drosophila melanogaster) | FRT42B didum[88] | Anne Ephrussi lab (EMBL); DOI: 10.1016/j.cub.2009.04.062 | ||
| Genetic reagent (Drosophila melanogaster) | UASp-MyoV∆1017–1114 aa-FLAG (attP64) | Generated in this study | ||
| Genetic reagent (Drosophila melanogaster) | vasa-Gal4 (III) | Allan Spradling lab (Carnegie Institution for Science);DOI: 10.1534/genetics.118.300874 | ||
| Genetic reagent (Drosophila melanogaster) | UASp-EMTB-3XTagRFP (III) | Generated in this study | ||
| Genetic reagent (Drosophila melanogaster) | UASp-GFP-Patronin (II) | Uri Abdu lab (Ben Gurion University) | ||
| Genetic reagent (Drosophila melanogaster) | UASp-Klp10A-GFP-SspB (II) | Generated in this study | ||
| Genetic reagent (Drosophila melanogaster) | UASp-LifeAct-HA-SsrA (II) | Generated in this study | ||
| Genetic reagent (Drosophila melanogaster) | maternal α-tubulin67-RFP-Staufen (III) | Daniel St Johnston lab (University of Cambridge); DOI: 10.1083/jcb.201103160 | ||
| Genetic reagent (Drosophila melanogaster) | vasa-Gal4 (II) | Yukiko Yamashita lab (University of Michigan) | ||
| Genetic reagent (Drosophila melanogaster) | UASp-KHC576-TagRFP-FKBP (III) | Generated in this study | ||
| Genetic reagent (Drosophila melanogaster) | UASp-MyoVHMM-GFP-FRB (II) | Generated in this study | ||
| Genetic reagent (Drosophila melanogaster) | Jupiter-GFP | Yale GFP Protein Trap Database (ZCL2183); DOI: 10.1073/pnas.261408198 | ||
| Genetic reagent (Drosophila melanogaster) | UASp-tdEOS2-alpha-tubulin84B (II) | DOI: 10.1016/j.cub.2013.04.050 | BDSC: #51313 | |
| Cell line (Spodoptera frugiperda) | Sf9 cells | Invitrogen(Thermo fisher); DOI: 10.1074/jbc.M113.499848; DOI: 10.7554/eLife.32871 | Maintained in J. Sellers lab; used for recombinant baculovirus expression | |
| Antibody | Mouse anti-Drosophila Staufen antibody | Chris Q. Doe lab (University of Oregon); DOI: 10.1073/pnas.1522424113; DOI: 10.1083/jcb.201709174 | IF (1:50) | |
| Antibody | Fluorescein (FITC) AffiniPure Goat Anti-Mouse IgG (H+L) | Jackson ImmunoResearch | Cat# 115-095-062 | IF (1:100) |
| Antibody | Rhodamine (TRITC)-AffiniPure Goat Anti-Mouse IgG (H+L) | Jackson ImmunoResearch | Cat# 115-025-003 | IF (1:100) |
| Antibody | 647-conjugated anti-tubulin nanobody (llamas/E. coli) | Helge Ewers lab (Freie Universität Berlin); DOI: 10.1038/ncomms8933 | IF (1:50) | |
| Chemical compound, drug | Rhodamine-labeled phalloidin | Thermo Fisher Scientific | Cat # R415 | IF (1:5000) |
| Chemical compound, drug | Colcemid | Cayman Chemical | Item No. 15364 | 200 μM |
| Chemical compound, drug | Rapalog (A/C Heterodimerizer) | Clontech/Takara | Cat# 635055 | 10 μM |
| Software, algorithm | A custom MatLab program that normalized the distance and fluorescent signal in the plot profiles | David Kirchenbüechler (CAM, Northwestern University); DOI: 10.1083/jcb.201709174 |