Video 1. Pseudovideo recording of the GFP signals of the intracellular Ca²⁺ sensor GCaMP5G after FSS stimulation.

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Images were continuously taken at time intervals of 5 s, and this pseudovideo was made from the first parallel 30 images. Left: Ad-Cre virus infected primary BMSCs from the control PC::G5-tdT mice; Right: Ad-Cre virus infected primary BMSCs from the Piezo1^fl/fl; PC::G5-tdT mice.