Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (M. musculus) | Piezo1 | Gene ID: 234839 | ||
| Gene (M. musculus) | Piezo2 | Gene ID: 667742 | ||
| Strain, strain background (C57BL/6J, Female and male) | C57BL/6J Wild type mice | Jackson laboratory | Cat# JAX:000664, RRID:IMSR_JAX:000664 | |
| Strain, strain background (C57BL/6J, Female and male) | Piezo1-tdTomato mice | Jackson laboratory | Cat# JAX:029214, RRID:IMSR_JAX:029214 | |
| Strain, strain background (C57BL/6J, Female and male) | Piezo1fl/fl mice | Jackson laboratory | Cat# JAX:029213, RRID:MSR_JAX:029213 | |
| Strain, strain background (C57BL/6J, Female and male) | Piezo2fl/fl mice | Jackson laboratory | Cat# JAX:027720, RRID:IMSR_JAX:027720 | |
| Strain, strain background (C57BL/6J, Female and male) | Piezo2-GFP-IRES-Cre mice | Jackson laboratory | Cat# JAX:027719, RRID:IMSR_JAX:027719 | |
| Strain, strain background (C57BL/6J, Female and male) | Ai9 mice | Jackson laboratory | Cat# JAX:007909, RRID:IMSR_JAX:007909 | |
| Strain, strain background (C57BL/6J, Female and male) | Prrx1-Cre mice | Jackson laboratory | Cat# JAX:005584, RRID:IMSR_JAX:005584 | |
| Strain, strain background (C57BL/6J, Female and male) | Sp7-GFP::Cre mice | Jackson laboratory | Cat# JAX:006361, RRID:IMSR_JAX:006361 | |
| Strain, strain background (C57BL/6J, Female and male) | PC::GCaMP5G-tdTomato mice | Jackson laboratory | Cat# JAX:024477, RRID:IMSR_JAX:024477 | |
| Cell line (Homo sapiens) |
HEK 293T | ATCC | Cat# ACS-4500, RRID:CVCL_4V93 | |
| Transfected construct (M. musculus) |
mPiezo1 siRNA | Sigma-Aldrich | Cat# SASI_ Hs01_00208584 |
|
| Transfected construct (M. musculus) |
siRNA Universal Negative Control | Sigma-Aldrich | Cat# SIC001 | |
| Biological sample (M. musculus) |
Primary BMSCs | Freshly isolated from M. musculus | ||
| Antibody | Anti-RFP (Rabbit polyclonal) |
Rockland | Cat# 600-401-379, RRID:AB_2209751 | 1:200 (IHC), 1:1000 (WB) |
| Antibody | Anti-Sp7 (Rabbit polyclonal) |
Abcam | Cat# ab22552, RRID:AB_2194492 | 1:600 (IHC), 1:2000 (WB) |
| Antibody | Anti-SO9 (Rabbit polyclonal) |
Millipore | Cat# AB5535, RRID:AB_2239761 | 1:500 (IHC) |
| Antibody | Anti-OPN (Goat polyclonal) |
R and D systems | Cat# AF808, RRID:AB_2194992 | 1:200 (IHC) |
| Antibody | Anti-Ctsk (Rabbit polyclonal) |
Abclonal | Cat# A1782, RRID:AB_2763824 | 1:100 (IHC) |
| Antibody | Anti-Trap (Rabbit polyclonal) |
Abclonal | Cat# A2528, RRID:AB_2764419 | 1:100 (IHC) |
| Antibody | Anti-BrdU (mouse monoclonal) |
Biolengd | Cat# 317902, RRID:AB_604040 | 1:100 (IHC) |
| Antibody | Anti-Col10a1 (Rabbit polyclonal) | Abclonal | Cat# A6889, RRID:AB_2767448 | 1:100 (IHC) |
| Antibody | Anti-YAP/TAZ (Rabbit monoclonal) | CST | Cat# 8418, RRID:AB_10950494 | 1:300 (IF), 1:200 (IHC), 1:1000 (WB) |
| Antibody | Anti-β-catenin (mouse monoclonal) | BD Biosciences | Cat# 610154, RRID:AB_397555 | 1:400 (IHC), 1:2000 (WB) |
| Antibody | Anti-β-catenin (Rabbit polyclonal) | Abclonal | Cat# A0316, RRID:AB_2757122 | 1:1000 (WB) |
| Antibody | Anti-NFAT2 (Rabbit polyclonal) | Abclonal | Cat# A1539, RRID:AB_2762296 | 1:200 (IF), 1:100 (IHC), 1:1000 (WB) |
| Antibody | Anti-Phospho-YAP (Ser127) (Rabbit polyclonal) | CST | Cat# 4911, RRID:AB_2218913 | 1:1000 (WB) |
| Antibody | Anti-Phospho-β-catenin (Thr41/Ser45) (Rabbit polyclonal) | CST | Cat# 9565, RRID:AB_331731 | 1:1000 (WB) |
| Antibody | Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb | CST | Cat# 8814S, RRID:AB_11127203 | 1:1000 (WB) |
| Antibody | β-Actin (8H10D10) Mouse mAb | CST | Cat# 3700, RRID:AB_2242334 | 1:50,000 (WB) |
| Antibody | Anti-LATS1 (Goat polyclonal) | Santa Cruz | Cat# sc-9388, RRID:AB_2133367 | 1:1000 (WB) |
| Antibody | Anti-Phospho-LATS1 (Thr1079) (Rabbit monoclonal) | CST | Cat# 8654, RRID:AB_10971635 | 1:1000 (WB) |
| Antibody | Anti-FAK (mouse monoclonal) | Santa Cruz | Cat# sc-271126, RRID:AB_10614323 | 1:500 (WB) |
| Antibody | Anti-Phospho-FAK (pY397) (mouse monoclonal) | BD Biosciences | Cat# 611806, RRID:AB_399286 | 1:1000 (WB) |
| Antibody | Anti-Mst1 (Rabbit monoclonal) | CST | Cat# 14946, RRID:AB_2798654 | 1:1000 (WB) |
| Antibody | Anti-Phospho-Mst1 (Thr183) (Rabbit monoclonal) | CST | Cat# 49332, RRID:AB_2799355 | 1:1000 (WB) |
| Antibody | Anti-RUNX2 (Rabbit polyclonal) | ABclonal | Cat# A2851, RRID:AB_2764676 | 1:1000 (WB) |
| Antibody | Anti-CTGF (Rabbit polyclonal) | ABclonal | Cat# A11067, RRID:AB_2758390 | 1:1000 (WB) |
| Antibody | Anti-Calcineurin (Rabbit polyclonal) | Abclonal | Cat# A1063, RRID:AB_2758155 | 1:1000 (WB), 1:200 (IP) |
| Antibody | Anti-Flag (M2) (mouse monoclonal) | Sigma | Cat# F1804, RRID:AB_262044 | 1:2000 (WB), 1:1000 (IP) |
| Antibody | Anti-HA (3F10) (Rat monoclonal) | Roche | Cat# 11867423001, RRID:AB_390918 | 1:1000 (WB) |
| Antibody | Anti-c-Myc (9E10) (Mouse monoclonal) | Santa Cruz | Cat# sc-40, RRID:AB_627268 | 1:1000 (WB) |
| Antibody | Anti-GAPDH (Rabbit monoclonal) | CST | Cat# 5174, RRID:AB_10622025 | 1:3000 (WB) |
| Antibody | Anti-DIG-AP conjugate (Sheep) | Roche | Cat# 11093274910, RRID:AB_514497 | 1:500 (ISH) |
| Antibody | Alexa Fluor 488 donkey anti-mouse (polyclonal) | Life Technologies | Cat# A-21202, RRID:AB_141607 | 1:500 (IHC) |
| Antibody | Alexa Fluor 488 donkey anti-rabbit (polyclonal) | Life Technologies | Cat# A-21206, RRID:AB_2535792 | 1:500 (IHC) |
| Antibody | Alexa Fluor 488 donkey anti-goat (polyclonal) | Life Technologies | Cat# A-11055, RRID:AB_2534102 | 1:500 (IHC) |
| Antibody | Alexa Fluor 568 donkey anti-mouse (polyclonal) | Life Technologies | Cat# A10037, RRID:AB_2534013 | 1:500 (IHC) |
| Antibody | Alexa Fluor 568 donkey anti-rabbit (polyclonal) | Life Technologies | Cat# A10042, RRID:AB_2534017 | 1:500 (IHC) |
| Antibody | Alexa Fluor 568 donkey anti-goat (polyclonal) | Life Technologies | Cat# A-11057, RRID:AB_142581 | 1:500 (IHC) |
| Antibody | ECL Donkey anti-rabbit | GE Healthcare Life Science | Cat# NA9340-1ml, RRID:AB_772191 | 1:5000 (WB) |
| Antibody | ECL Sheep anti-mouse | GE Healthcare Life Science | Cat# NA9310-1ml, RRID:AB_772193 | 1:5000 (WB) |
| Antibody | Bovine anti-goat IgG HRP | Santa Cruz | Cat# sc-2350, RRID:AB_634811 | 1:5000 (WB) |
| Antibody | Donkey Anti-Rat IgG Antibody | Sigma | Cat# AP189P, RRID:AB_11214462 | 1:5000 (WB) |
| Recombinant DNA reagent | pcDNA3.0-YAP-HA (plasmid) | This paper | ||
| Recombinant DNA reagent | pcDNA3.0-NFATc1-Flag (plasmid) | This paper | ||
| Recombinant DNA reagent | pcDNA3.0-β-catenin-Myc (plasmid) | This paper | ||
| Recombinant DNA reagent | pGL3-NFAT luciferase (plasmid) | Addgene | RRID:Addgene_17870 | |
| Recombinant DNA reagent | 8XGTIIC-luciferase (plasmid) | Addgene | RRID:Addgene_34615 | |
| Recombinant DNA reagent | Super 8X TOPFlash (plasmid) | Addgene | RRID:Addgene_12456 | |
| Recombinant DNA reagent | pTK-Renilla (plasmid) | Promega | Cat# E2241 | |
| Recombinant DNA reagent | pcDNA3.0-Calcineurin A-S (plasmid) | This paper | ||
| Sequence-based reagent | Primers listed in supplemental table | |||
| Commercial assay or kit | Gel Extraction Kit | Omega | Cat# D2500 | |
| Commercial assay or kit | RNAscope 2.5 HD Reagent Kit | ACD | Cat# 322350 | |
| Commercial assay or kit | RNAscope Probe Mm-Piezo1 | ACD | Cat# 500511 | |
| Commercial assay or kit | RNAscope Probe Mm-Ctgf |
ACD | Cat# 314541 | |
| Commercial assay or kit | 1-Step NBT/BCIP Substrate Solution | Life Technologies | Cat# 34042 | |
| Commercial assay or kit | Ion 550 Chip Kit | Life Technologies | Cat# A34541 | |
| Commercial assay or kit | Ion AmpliSeq Transcriptome Mouse Gene Expression Panel, Chef-Ready Kit | Life Technologies | Cat# A36412 | |
| Commercial assay or kit | Dual-Luciferase Reporter Assay | Promega | Cat# E1910 | |
| Commercial assay or kit | Lipofectamine 3000 | Life Technologies | Cat# L3000001 | |
| Commercial assay or kit | RAT/Mouse P1NP ELISA kit | Immunodiagnostic Systems Inc | Cat# AC33F1 | |
| Commercial assay or kit | Alexa Fluor 594 Tyramide SuperBoostTM Kit | Life Technologies | Cat# B40944 | |
| Commercial assay or kit | Masson’s Trichrome | Abcam | Cat# ab150686 | |
| Commercial assay or kit | TRAP staining kit | Sigma-Aldrich | Cat# 387A | |
| Commercial assay or kit | TUNEL kit | Life Technologies | Cat# C10617 | |
| Commercial assay or kit | FAST SYBR Green Master Mix | Life Technologies | Cat# 4385612 | |
| Commercial assay or kit | High-Capacity cDNA Reverse Transcription Kit | Life Technologies |
Cat# 4374966 | |
| Chemical compound, drug | DIG RNA labeling mix | Roche | Cat# 11277073910 | |
| Chemical compound, drug |
Protector RNase inhibitor | Roche | Cat# 03335399001 | |
| Chemical compound, drug | T7 RNA polymerase | Roche | Cat# 10881767001 | |
| Chemical compound, drug | T3 RNA polymerase | Roche | Cat# 11031163001 | |
| Chemical compound, drug | LiCl | Sigma-Aldrich | Cat# L4408 | |
| Chemical compound, drug | Phusion High-Fidelity DNA Polymerase | New England Biolabs | Cat# M0530 | |
| Chemical compound, drug | Proteinase K | Sigma-Aldrich | Cat# P2308 | |
| Chemical compound, drug | Glutaraldehyde solution | Sigma-Aldrich | Cat# G5882 | |
| Chemical compound, drug | Yeast tRNA | Sigma-Aldrich | Cat# R8759 | |
| Chemical compound, drug | Blocking reagent | Roche | Cat# 11096176001 | |
| Chemical compound, drug | BM-purple | Roche | Cat# 11442074001 | |
| Chemical compound, drug | Alizarin Red S | Sigma-Aldrich | Cat# A5533 | |
| Chemical compound, drug | Alcian Blue | Sigma-Aldrich | Cat# A9186 | |
| Chemical compound, drug | RNAzol RT | Sigma-Aldrich | Cat# R4533 | |
| Chemical compound, drug | Calcein | Sigma-Aldrich | Cat# C0875 | |
| Chemical compound, drug | Sliver nitrate | Sigma-Aldrich | Cat# S8157 | |
| Chemical compound, drug | Sodium thiosulfate | Sigma-Aldrich | Cat# 72049 | |
| Chemical compound, drug | BGjb medium | Life Technologies | Cat# 12591038 | |
| Chemical compound, drug | Yoda1 | TOCRIS | Cat# 5586 | |
| Chemical compound, drug | Cyclosporin A | LC Laboratories | Cat# LC-C-6000 | |
| Chemical compound, drug | Gadolinium (III) chloride | Sigma-Aldrich | Cat# 439770 | |
| Chemical compound, drug | β-glycerophosphate | Sigma-Aldrich | Cat# G9422 | |
| Chemical compound, drug | L-ascorbic acid | Sigma-Aldrich | Cat# A5960 | |
| Chemical compound, drug | Sulfo-SANPAH | Life Technologies | Cat# 22589 | |
| Chemical compound, drug | Collagen I, Rat tail | Corning | Cat# 354236 | |
| Chemical compound, drug | BIO | Sigma-Aldrich | Cat# B1686 | |
| Chemical compound, drug | 40% (w/v) acrylamide stock solution | Sigma-Aldrich | Cat# A4058 | |
| Chemical compound, drug | 2% (w/v) bis-acrylamide stock solution | Sigma-Aldrich | Cat# M1533 | |
| Software, algorithm | GraphPad Prism | GraphPad Prism | RRID:SCR_002798 | |
| Software, algorithm | ImageJ | ImageJ | RRID:SCR_003070 | |
| Software, algorithm | DAVID | DAVID | RRID:SCR_001881 | |
| Software, algorithm | Photoshop | Adobe | RRID:SCR_014199 | |
| Software, algorithm | Transcriptome Analysis Console | Life Technologies | RRID:SCR_016519 |