TABLE 2.

Tolerance phenotypes for S. aureus USA300_TCH1516 and medium-adapted evolved populations on CA-MHB and RPMI+^a

Ancestor strain and strain	ALE no.	Initial growth rate (h−1)	Starting nafcillin concn (μg/ml)	Final growth rate (h−1)	Final nafcillin concn (μg/ml)	No. of flasks	CCD × 1012
RPMI+ TALE (SNFR)
STR 1	7	1.17 ± 0.02	0.013	0.83 ± 0.12	65.52	184	15.2
	9	1.20 ± 0.03	0.013	0.83 ± 0.08	50.4	174	13.4
STR 4	13	1.23 ± 0.08	0.013	0.85 ± 0.09	83.16	191	14.5
	15	1.17 ± 0.08	0.013	0.83 ± 0.06	57.96	177	14.2
	17	1.04 ± 0.07	0.013	0.74 ± 0.11	65.52	172	13.6
STR 5	19	1.11 ± 0.1	0.013	0.87 ± 0.14	52.92	166	12.8
	21*	1.19 ± 0.04	0.013	0.99 ± 0.08	4.32	117	8.5
	23	1.22 ± 0.08	0.013	0.89 ± 0.14	57.96	171	13.6
CA-MHB TALE (SNFM)
STM 1	7	0.79 ± 0.07	0.5	0.77 ± 0.17	61.2	72	3.94
	11	0.90 ± 0.12	0.5	0.87 ± 0.07	80.33	75	4.08
STM 2	13	0.94 ± 0.11	0.5	0.70 ± 0.03	61.2	68	3.75
	15	0.87 ± 0.14	0.5	0.76 ± 0.13	61.2	70	3.81
STM 3	19	0.97 ± 0.16	0.5	0.88 ± 0.08	61.2	74	3.95
	23	0.93 ± 0.11	0.5	0.93 ± 0.06	61.2	74	4.16

^aPopulation growth rates for independent replicates were calculated by averaging the initial and final three flasks of the medium adaptation ALEs. An asterisk indicates premature end to experiment due to technical errors.