Figure 4.

Excess androgens in polycystic ovary syndrome (PCOS) hormone condition increased proliferation of cells in endometrial organoids. (A) Endometrial organoids were cultured in normal (left) and PCOS (right) conditions as described in Fig. 1. Cell proliferation was detected by immunofluorescent staining of Ki67 in epithelial cells (dual stained with E-cadherin in pink). (B) Ki67+ cells were quantified in endometrial organoids treated with normal or PCOS hormones. Organoids were obtained from 4 patients. (C) Organoids were treated with normal or PCOS hormones using R1881 as the androgen and the Ki67+ cells were quantified. Organoids were obtained from 3 patients. Data were analyzed using unpaired Student’s t-test based on n = number of organoids.