Figure 1: Batf is expressed by lung ILC2 cells and is predicted to regulate distinct gene sets in KLRG1^pos populations.

WT C57BL/6 mice were injected intraperitoneally (i.p.) with IL-33 daily for 4 days and pulmonary KLRG1^neg (non-ILC2s) and KLRG1^pos (ILC2s) were sorted on day 5 and used for RNA-sequencing. (A) Diagonal scatter plot comparing differentially expressed genes (padj <0.05) among KLRG1^neg and KLRG1^pos cells. (B) Heat map of ILC2-related genes as well as S1P receptor genes upregulated in KLRG1^pos ILCs *p≤0.05. (C) Transcription factors predicted by PASTAA analysis to be involved in regulating genes that were enriched among KLRG1^pos, compared to KLRG1^neg, populations. (D) Normalized enrichment scores of the predicted transcription factors as assessed with gene set enrichment analysis of KLRG1^pos ILC2s compared to the KLRG1^neg population. Binding motifs as reported by TRANSFAC are also included in the table where S=C or G, W=A or T, R=A or G, Y=C or T, M= A or C, and N=any base. RNA-sequencing analysis was compiled from three separate experiments with n=3-6 mice per group.