Figure 6: Appearance of KLRG1^high pulmonary iILC2s in the lung is blocked by FTY720.

(A-C) RNA sequencing was performed on KLRG1^pos and KLRG1^neg Lin⁻CD90⁺ lung cells from mice treated with IL-33 as described in Figure 1. (A) Gene Ontology Molecular Function and (B) KEGG Pathway analyses highlighting gene groups and pathways that are significantly associated with genes that are at increased at least two-fold in KLRG1^pos relative to KLRG1^neg populations, as performed in Figure 5. Gene groups and pathways associated with cell trafficking are highlighted in red. (C) Heatmap of chemokine receptor expression in indicated populations from three separate RNA-sequencing experiments. Significant differential expression is denoted by an asterisk. (D-G) IL13^yetcre13 mice were infected with N. brasiliensis on day 0 and given saline control or FTY720 on days 0, 2, and 4 after infection. Pulmonary Lin⁻ CD90⁺ ILC2s were assessed on day 5 by flow cytometry. (D) Representative contour plots of the frequency of KLRG1^mid and KLRG1^high lung ILC2s. (E) Combined frequency and number of KLRG1^high cells, n=3 from a single experiment representative of 3 independent experiments. (F) Contour plots of YFP (IL-13mRNA) expression within the KLRG1⁺ subset of Lin⁻CD90⁺ ILC2s. (G) Combined frequency and number of YFP⁺ Lin⁻CD90⁺KLRG1^high cells, n=3 from a single experiment representative of 3 independent experiments. *p≤0.05, **p≤0.01 as determined by two-tailed unpaired t test.