. 2017 Aug 8;145(13):2717–2726. doi: 10.1017/S0950268817001224

Table 1.

Primers used for PCR

Sr. no.	Agent	Primer	Sequence (5′−3′)	PCR conditions	Product size (base pair)	Reference
1	Bovine coronavirus (N)	BCoVF	CCGATCAGTCCGACCAATC	Initial denaturation 95 °C (5 min), 40 cycles of denaturation 94 °C (30 s), annealing 55 °C (1 min), elongation 72 °C (1 min) and final elongation at 72 °C (7 min)	406	Tsunemitsu et al. (1999) [60]
1	Bovine coronavirus (N)	BCoVR	AGAATGTCAGCCGGGGTAT		406	Tsunemitsu et al. (1999) [60]
2	Escherichia coli K99⁺ (K99)	K99F	GCGACTACCAATGCTTCTGCGAATAC		230	Cho et al. (2010) [25]
2	Escherichia coli K99⁺ (K99)	K99R	GAACCAGACCAGTCAATACGAGCA		230	Cho et al. (2010) [25]
3	Salmonella (16SrDNA)	16 SF	TGTTGTGGTTAATAACCGCA		575	Lin et al. (1996) [61]
3	Salmonella (16SrDNA)	16SR	CACAAATCCATCTCTGGA		575	Lin et al. (1996) [61]
4	Cryptosporidium (COWP)	BB-3F	GCGAAGATGACCTTTTGATTTG		194	Balatbat et al. (1996) [48]
4	Cryptosporidium (COWP)	BB-4R	AGGATTTCTTCTTCTGAGGTTCC		194	Balatbat et al. (1996) [48]
5	Clostridium perfringens	Clostri. F	AAAGATGGCATCATCATTCAAC	Initial denaturation 94 °C (5 min), 35 cycles of denaturation 94 °C (1 min), annealing 53 °C (1 min), elongation 72 °C (1 min) and final elongation at 72 °C (7 min)	279	Yoo et al. (1997) [62]
5	Clostridium perfringens	Clostri. R	TACCGTCATTATCTTCCCCAAA		279	Yoo et al. (1997) [62]