Fig. 4.

Unconventional Xbp1 mRNA Splicing is changed in schizophrenia. a Diagram illustrating the predicted mRNA products of Xbp1 and their digestion by PstI. uXbp1 (band 1) contain a Pstl site that leads to cleavage into two transcripts (bands 3 and 4), although some of this population appears to be resistant to Pstl digestion. The unique PstI site is removed when Xbp1 is spliced by IRE1α (sXbp1), resulting in this band (2) being unaffected by Pstl digestion. b Representative resultant mRNA digested with PstI follow by emulsion cDNA and PCR amplification, and analyzed by 2% agarose gel electrophoresis to visualize the spliced (s) and unspliced (u) products. c The ratio of sXbp1/ uXbp1 mRNA levels was increased in paired schizophrenia (SCZ) and comparison (COMP) subjects. Data are expressed as means ± SEM. **p < 0.01