Table 3.
Total lysine content of axonal proteins. Protein reactivity of 1,2-DAB and formation of high molecular weight adducts correlated with lysine content. Both in vivo and in vitro, rat neurofilament (NF) heavy chain (NF-H) and NF-M formed polymers more readily than either NF-L or tubulin. Other 1,2-DAB protein targets involved in anterograde axonal transport included rat microtubule-associated tau, the rat motor proteins kinesin and dynein, and the rabbit glycolytic enzymes glyceraldehyde phosphate dehydrogenase (GAPDH: 8% lysine) and lactate dehydrogenase (LDH: 7–8% lysine). Stathmin, which contains a very high lysine content, was not tested. Data from Kim et al.48
| Axonal Protein | Percentage Lysine Content |
|---|---|
| (Stathmin | 15.5) |
| Neurofilament Heavy Chain (NF-H) | 13–17 |
| Neurofilament Medium Chain (NF-M) | 11–15 |
| Neurofilament Light Chain (NF-L) | 6–7 |
| Beta-Tubulin | 3–4 |
| Kinesin | 8–12 |
| Dynein | 8 |
| Tau | 7–8 |