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. 2019 Dec 31;58(2):335–347. doi: 10.1007/s10528-019-09945-x

Table 2.

Primers used for plasmid construction

Gene name	Primer pair	Primer sequence (5′–3′)	Product (bp)
POU2F3-1	POU2F3-1-P2-F	CCGGAATTC^a TGGTGAATCTGGAACCCATGCA	1305
	POU2F3-1-P2-R	GCTCGAG^b TCAGTGGAGGTACGTGGGA
POU2F3-2	POU2F3-2-P2-F	CCGGAATTCTGCTCAAAGTGCTCTCAGCC	1206
	POU2F3-2-P2-R	GCTCGAGTCAGTGGAGGTACGTGGGATGACT
POU2F3-3	POU2F3-3-P2-F	CCGGAATTCTGGGAAAGCTGTATGGAAACGAC	681
	POU2F3-3-P2-R	GCTCGAGTCAGTGGAGGTACGTGGGATGACT
KRT14	KRT14-P1-F	GGGTACC^c CTAGGGTCGGTGCTAAGGGCT	662
	KRT14-P1-R	CAAGCTT^d GTCGGCAGGGAAGAGAAAGGT
MMP19	MMP19-P1-F	GGGTACCCCACCCTTCGTTCTTTCGTC	519
	MMP19-P1-R	CAAGCTTGAGTTTGTGCCCTCCGCTCT

^aGAATTC: EcoRI site

^bCTCGAG: XhoI site

^cGGTACC: KpnI site

^dAAGCTT: HindIII site