Fig. 2.

Cytokine-induced IFN-γ mRNA transcription in anti-CD20-experienced cells. a Purified experienced NK cells were obtained and stimulated with cytokines as in Fig. 1a. After 18 h, total RNA was extracted from untreated (none) or cytokine-activated NK cells and IFN-γ mRNA levels were measured by RT-qPCR. The fold change expression relative to untreated populations (set to 1) after normalizing with the GAPDH endogenous control is reported in the bar graph. Data (mean ± SEM) from n = 6 donors of three independent experiments are shown, *p = 0.0313. b Purified experienced NK cells were stimulated as indicated for 30 min. Western blot analysis of equal amounts of total lysates (4 × 10⁵cells/point) were probed with anti-phospho-STAT4 (p-STAT4) followed by anti-STAT4 mAbs. All lanes were from the same experiment but were not contiguous. One representative experiment out of three performed is shown (left panels). Densitometric analysis of phosphorylated STAT4 (p-STAT4) normalized to total STAT4 in IL-12-stimulated samples is represented. Data (means ± SEM) from n = 3 donors (right panel)