Fig. 3.

Obinutuzumab-mediated CD16 ligation upregulates miR-155 levels in NK cells from healthy donors and CLL patients. a Primary cultured NK cells were stimulated (2:1) for 18 h with biotinylated rituximab (RTX-exp)-, obinutuzumab WT (GA101 WT-exp)-, obinutuzumab (GA101-exp)-opsonized, not opsonized Raji (Ctrl-exp) or left unstimulated and immunomagnetically purified by negative selection, or b NK cells from three untreated CLL patients were stimulated with biotinylated rituximab (RTX-exp)-, obinutuzumab (GA101-exp)-opsonized autologous leukemia or left unstimulated and purified as in a. Relative miR-155 (a, b) or miR-29a (a) levels were measured by RT-qPCR. Bar graphs depict the fold change expression relative to the unstimulated population (set to 1) after normalizing with the RNU-44 endogenous control. a Data (mean ± SEM) from n = 17 donors from five independent experiments are shown. ****p < 0.0001