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. 2020 Apr 1;10:5742. doi: 10.1038/s41598-020-62768-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of thrombin inhibitors with in-line pressure sensor. (A) Photograph showing experimental setup that consists of a syringe pump (1) connected to an in-line pressure sensor (2), microfluidic device (3), pressure display monitor (4), and laptop computer (5). The syringe pump pulls the blood at a constant flow rate through the outlet of the microfluidic device and the changes in fluid pressure caused by channel occlusion are detected by the pressure sensor which sends the pressure data to the laptop computer for display. (B) Conceptual illustration of experimental set up and measurement of clotting time from pressure data. (C) Graph showing mean pressure trace measured in the device when re-calcified citrated whole blood treated with varying heparin doses (0, 0.5, 1.0 IU/mL) was perfused (mean shear rate, 1,200 1/s) through collagen coated devices (n = 3 donors, 1 replicate per experiment) (D) Clotting time (CT) derived from heparin pressure trace; (no clotting, **P < 0.01, n = 3 donors). (E) Graph showing mean pressure trace measured in the device when re-calcified citrated whole blood treated with varying bivalirudin doses (0, 50, 75 ng/mL) was perfused (mean shear rate, 1,200 1/s) (n = 3 donors, 1 replicate per experiment). (F) Clotting time (CT) derived from bivalirudin pressure trace; (no clotting, **P < 0.01, n = 3 donors).