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. 2020 Apr 1;10:5781. doi: 10.1038/s41598-020-62516-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluidic culture of different breast cancer cell lines. (A) Schematic of fluidic device with tumoroid (side view). Cancer cells are seeded into one of the fluidic channels (channel B) and the channel subsequently sealed. Growth media is introduced into the chamber via peristaltic pump through channel A. (B) Phase images of tumoroid channel for triple negative breast cancer cell lines: SUM149 (first row); BT549 (second row); MDA-MB-231 (third row) and HCC1937 (last row). (C) Measurements of observed invasion distance of SUM149, BT549, MDA-MB-231 and HCC1937 over time. n = 3 *p < 0.05.