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. 2020 Apr 1;10:5781. doi: 10.1038/s41598-020-62516-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemistry of tumoroids for markers of invasion. SUM149 (A) and PDX #2 (B) tumoroids were stained for KRT14, Ki67, ADAM19, BEX1 and CDH13. 3D surface plots of staining intensity (represented in the middle panel of each row) while quantification of staining intensity in tumoroid (T) or invading cells (I) indicated as the percentage of cells that display stronger staining (represented in the third panel of each row). n = 3 *p-value < 0.05; ns = not significant.