Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 1;11(4):214. doi: 10.1038/s41419-020-2405-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Knockdown of c-Myc in Rv1 cells reduced mRNA levels of indicated DDR genes. Cells were transduced with pLKO.1 control or c-Myc shRNAs for 48h. RNAs were analyzed by qRT-PCR for the indicated genes. b Knockdown of c-Myc in the JMJD1A-knockdown Rv1 cells did not further reduce the expression of DDR genes. Knockdown of JMJD1A alone or knockdown of both JMJD1A and c-Myc was performed on Rv1 cells for 48h. Cells were analyzed by qRT-PCR for the indicated DDR genes. c Coimmunoprecipitation between JMJD1A and c-Myc in Rv1 cells. Left panel: JMJD1A was immunoprecipitated from Rv1 cells, and analyzed by western blotting for the coprecipitation of c-Myc. Right panel: c-Myc was immunoprecipitated from Rv1 cells, and analyzed by western blotting for the coprecipitation of JMJD1A. d Coprecipitation of myc-JMJD1A with Flag-c-Myc but not with Flag-Max. myc-JMJD1A was coexpressed with Flag-c-Myc or Flag-Max in 293T cells. Cell lysates were subjected to immunoprecipitation (IP) with anti-Flag M2 beads. Bound proteins were analyzed by western blotting with myc or Flag antibodies. e Interaction of N-terminal transactivation domain of c-Myc with JMJD1A. myc-JMJD1A was coexpressed with the Flag-tagged truncation mutants of c-Myc and analyzed by Flag IP as described in (d). f Interaction of N-terminal or C-terminal half of JMJD1A with c-Myc. HA-tagged c-Myc was coexpressed with the Flag-tagged N-terminal half (N) or C-terminal half (C) of JMJD1A, and analyzed by the Flag IP. g Direct binding between purified c-Myc and JMJD1A. GST or GST-c-Myc bound on the glutathione agarose was incubated with the recombinant Flag-JMJD1A. The bound proteins were analyzed by western blotting with GST or Flag antibodies. h Enrichment of JMJD1A and c-Myc on the E box sites of indicated DDR genes in Rv1 cells. Cells were analyzed by ChIP using antibodies for control IgG, JMJD1A or c-Myc. The precipitated chromatin fragments were analyzed by qPCR with primers targeting the E box region of indicated DDR genes. Data were calculated as the percentage of input. i−k c-Myc knockdown in Rv1 cells reduced the enrichment of JMJD1A and increased the enrichment of H3K9me2 at the E box region of XRCC6 (i), PRKDC (j) or BARD1 (k) gene. l−n JMJD1A knockdown in Rv1 cells increased the enrichment of H3K9me2 and decreased the enrichment of c-Myc at the E box region of indicated DDR genes. *(p < 0.05), **(p < 0.01).