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. 2020 Apr 1;5:15. doi: 10.1038/s41525-020-0122-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Human phosphokinase array screening of changes in signaling pathways in HCC cells upon alteration of MACROD2 expression. b Western blot to validate the expression of p-GSK-3β and β-catenin in HCC cells upon alteration of MACROD2 expression. c Results of dual-luciferase assays performed 48 h after transfection of the indicated cells with TOPflash or FOPflash and Renilla pRL-TK plasmids. Reporter activity was normalized to Renilla luciferase activity. **P < 0.01. d Representative images of xenograft-derived tumor sections show p-GSK-3β and β-catenin expression. Scale bars = 100 μm. e Immunofluorescence staining showing subcellular β-catenin localization in the indicated cells. Data are shown as the mean ± SD and are representative of three independent experiments.