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. 2020 Apr 1;10:5770. doi: 10.1038/s41598-020-62404-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of the solid phase PCR process. The PDMS/aluminum composite is formed (a,b). PCR mix and DNA template are digitally filled into the cavities (c). The chip is sealed with the primer coupled slide (d). The main PCR components nNTPs, polymerase, DNA template and primer (d.i). In order immobilise the PCR product, the forward primer is also covalently attached on the surface (d.ii). After the PCR process the chip is opened and the double stranded monoclonal DNA microarray is ready for further use (e). For the visualization of the DNA spots, the double stranded array is dehybridized and subsequently hybridized with a fluorescently labeled probe (f).