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. 2020 Apr 1;10:5770. doi: 10.1038/s41598-020-62404-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SCORE binding experiment using a generated DNA microarray. (a) Endpoint binding image of the DNA microarray. The brighter the signal, the more higher the binding signal is. (b) Detailed binding kinetic data for two DNA spots and one background spot (as indicated by arrows in (a)). The gray bars illustrate the time frames of analyte injections which are separated by washing steps. The analyte flushing sequence consists of a Thrombin step followed by an anti-Thrombin antibody step followed by a secondary antibody step. Furthermore, the binding steps are illustrated by small schematic pictures.