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SDS-PAGE analysis and pectin methylesterase activity assay. The expressed and purified fused protein was analyzed by 10% polyacrylamide gel. (Lane 1) Recombinant clone after induction with IPTG, (lane 2) transformed cells containing vector alone after induction with IPTG. (Lane 3) Purified fusion protein (FP). Pectin methylesterase activity assay was carried out by measuring the absorbance of the samples containing varying concentrations of the fused DUF538 protein at 550 nm
