Table 2.

Antifungal activity of Juniperus polycarpos var. turcomanica essential oil by two different methods

Fungi	Concentration (µLL−1)	Percent inhibition of mycelia growtha
		Method
		Agar dilution			Disc diffusion
		Day			Day
		1	3	6	1	3	6
Alternaria alternata	100	74.4 ± 2.72 bb	49.6 ± 8.76 d	27.7 ± 6.66 f	58.8 ± 2.50 b-e	43.5 ± 9.56 fg	27.5 ± 7.11 h
	200	100.0 ± 0.00 a	50.0 ± 4.22 d	39.4 ± 3.79 e	67.1 ± 6.14a-c	46.9 ± 3.56 ef	28.6 ± 6.18 h
	400	100.0 ± 0.00 a	75.9 ± 4.07 b	51.4 ± 2.41 d	71.8 ± 3.60 ab	57.7 ± 8.31c-e	31.1 ± 7.03gh
	800	100.0 ± 0.00 a	77.6 ± 3.54 b	65.6 ± 0.91 c	77.0 ± 6.09 a	61.1 ± 6.51b-d	49.4 ± 5.25 d-f
	Control (tween + alcohol)	3.7 ± 1.1 g	2.3 ± 1.2 g	0.0 ± 0.0 g	–	–	–
	Control	0.0 ± 0.0 g	0.0 ± 0.0 g	0.0 ± 0.0 g	0.0 ± 0.0 i	0.0 ± 0.0 i	0.0 ± 0.0 i
Macrophomina phaseolina	100	71.5 ± 4.03 d	56.9 ± 2.07 e	44.7 ± 4.29 g	100.0 ± 0.00 a	76.9 ± 7.88 de	65.0 ± 4.67 f
	200	87.0 ± 9.45 b	69.4 ± 5.77 cd	53.3 ± 2.41 f	100.0 ± 0.00 a	89.9 ± 1.04b	73.1 ± 3.67e
	400	100.0 ± 0.0 a	77.3 ± 4.73 c	62.8 ± 2.65 d	100.0 ± 0.00 a	100.0 ± 0.00 a	81.4 ± 2.46 cd
	800	100.0 ± 0.0 a	85.8 ± 4.47 b	69.2 ± 1.38 cd	100.0 ± 0.00 a	100.0 ± 0.00 a	85.0 ± 3.79 bc
	Control (tween + alcohol)	3.8 ± 1.2 h	2.2 ± 1.4 h	0.8 ± 0.5 h	–	–	–
	Control	0.0 ± 0.0 h	0.0 ± 0.0 h	0.0 k ± 0.0 h	0.0 ± 0.0 g	0.0 ± 0.0 g	0.0 ± 0.0 g
Curvularia fallax	100	73.7 ± 2.48 bc	49.3 ± 11.19 d	27.7 ± 6.66 f	100.0 ± 0.00 a	76.9 ± 7.88 de	65.0 ± 4.67 f
	200	100.0 ± 0.00 a	50.0 ± 4.22 d	39.4 ± 3.79 e	100.0 ± 0.00 a	89.9 ± 1.04 b	73.1 ± 3.67 e
	400	100.0 ± 0.00 a	73.4 ± 6.04 bc	53.9 ± 5.94 d	100.0 ± 0.00 a	100.0 ± 0.00 a	81.4 ± 2.46 cd
	800	100.0 ± 0.00 a	77.6 ± 3.54 b	66.6 ± 0.91c	100.0 ± 0.00 a	100.0 ± 0.00 a	85.0 ± 3.79 bc
	Control (tween + alcohol)	3.5 ± 1.4 g	2.2 ± 1.1 g	0.0 ± 0.0 g	–	–	–
	Control	0.0 ± 0.0 g	0.0 ± 0.0 g	0.0 ± 0.0 g	0.0 ± 0.0 g	0.0 ± 0.0 g	0.0 ± 0.0 g
Cytospora sacchari	100	100.0 ± 0.00 a	75.0 ± 4.07 c	65.6 ± 5.51d	72.8 ± 4.20 cd	63.5 ± 3.79 ef	48.7 ± 8.07 g
	200	100.0 ± 0.00 a	100.0 ± 0.00 a	75.6 ± 2.59 c	100.0 ± 0.00 a	71.5 ± 2.96 cd	59.2 ± 2.46 f
	400	100.0 ± 0.00 a	100.0 ± 0.00 a	83.6 ± 1.05 b	100.0 ± 0.00 a	75.3 ± 2.75 c	67.0 ± 1.06 de
	800	100.0 ± 0.00 a	100.0 ± 0.00 a	87.8 ± 2.38 b	100.0 ± 0.00 a	90.6 ± 1.92 b	75.8 ± 1.90 c
	Control (tween + alcohol)	4.1 ± 1.6 e	3.7 ± 1.3 ef	2.5 ± 1.2 ef	–	–	–
	Control	0.0 ± 0.0 f	0.0 ± 0.0 f	0.0 ± 0.0f	0.0 ± 0.0 h	0.0 ± 0.0 h	0.0 ± 0.0 h
Fusarium oxysporum	100	100.0 ± 0.00 a	70.0 ± 2.37 b	61.1 ± 0.89 b	100.0 ± 0.00 a	68.0 ± 3.33d	51.7 ± 2.67 f
	200	100.0 ± 0.00 a	77.9 ± 4.30 ab	70.0 ± 3.24 b	100.0 ± 0.00 a	76.6 ± 8.61c	59.2 ± 2.24 e
	400	100.0 ± 0.00 a	100.0 ± 0.00 a	80.6 ± 1.42 ab	100.0 ± 0.00 a	86.4 ± 2.91b	71.1 ± 2.84 cd
	800	100.0 ± 0.00 a	100.0 ± 0.00 a	65.6 ± 2.42 b	100.0 ± 0.00 a	90.4 ± 1.47 b	76.4 ± 1.43 d
	Control (tween + alcohol)	3.9 ± 1.3 c	3.6 ± 1.4 c	2.2 ± 1.1c	–	–	–
	Control	0.0 ± 0.0c	0.0 ± 0.0 c	0.0 ± 0.0 c	0.0 ± 0.0 g	0.0 ± 0.0 g	0.0 ± 0.0 g
Colletotricbum trichellum	100	100.0 ± 0.00 a	100.0 ± 0.00 a	100.0 ± 0.00 a	66.1 ± 6.93 bc	50.6 ± 4.16 ef	41.7 ± 7.98 f
	200	100.0 ± 0.00 a	100.0 ± 0.00 a	100.0 ± 0.00 a	71.3 ± 6.29 bc	62.9 ± 4.56 cd	54.8 ± 3.93de
	400	100.0 ± 0.00 a	100.0 ± 0.00 a	100.0 ± 0.00 a	74.6 ± 4.17 b	63.3 ± 6.11 cd	61.4 ± 1.39 cd
	800	100.0 ± 0.00 a	100.0 ± 0.00 a	100.0 ± 0.00 a	100.0 ± 0.00 a	66.2 ± 7.41 bc	67.2 ± 1.43bc
	Control (tween + alcohol)	4.5 ± 1.3 b	3.8 ± 1.2 c	2.7 ± 1.2 d	–	–	–
	Control	0.0 ± 0.0 e	0.0 ± 0.0 e	0.0 ± 0.0 e	0.0 ± 0.0 g	0.0 ± 0.0 g	0.0 ± 0.0 g

^aData are mean ± SD (standard deviation). (n = 4)

^bMeans comparison were done between different concentrations of Juniperus oil in various days for each fungus. Means with different letters about each fungus in each method are statistically significant at 5% level probability