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. 2020 Mar 26;11:272. doi: 10.3389/fphar.2020.00272

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Copyright © 2020 Huang, Ding, Zou, Lu, Wang and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of hAFMSCs was performed. (A) The expression levels of CD105, CD29, CD34, CD73, CD90, and CD73 in hAFMSCs were determined by flow cytometry. (B) hAFMSCs can differentiate into adipocytes (oil red), osteoblasts (alizarin red), and chondroblasts (alcian blue) under standard in vitro differentiating conditions. (C) The protein expressions of osteogenic (BSP, RUNX2), adipogenic (ADPF, PPARƳ), and chondrogenic (AGGRECAN, COL10A1) cells were tested in hAFMSCs and in HDF (human dermal fibroblast cell line). Scale bars = 10 μm. hAFMSCs = human amniotic fluid mesenchymal stem cells.