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hAFMSCs elevated ovary expression after injection into an OPA mouse model. (A) qPCR assays showed that hAFMSCs increased ovarian marker expression at four stages: PGC oogonia (FOXL2 and CYP19A1), follicle formation (MSH4 and STAG3), follicular growth (GDF9 and AMH), and follicular maturation (FSHR and BMP15). (B) Western blot assay showed that hAFMSCs increased ovarian marker expression at the four stages. The error bars indicate the SD; **, p < 0.01; ***, p < 0.001.
