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. 2020 Mar 26;11:229. doi: 10.3389/fphys.2020.00229

FIGURE 1.

FIGURE 1

Schematic representation of the different clock neurons in the Drosophila brain. The right brain hemisphere depicts the traditional division in different clock neurons including their classification in morning (M) and evening (E) neurons in reddish and bluish colors, respectively. Clock neurons that cannot be unequivocally assigned as M or E neurons are shown in gray. Note that the DN1p consist of a mixture of M and E neurons. The left-brain hemisphere depicts the M and E neurons in more detail and indicates in which specific neurons we rescued PER in per0 mutants (green edging). Due to limited gal4-drivers, we were not always able to restrict PER to only M or E neurons. For example, our M-DN driver (Clk4.1M-gal4) included also ∼2 DN1p that belong to the E-DN. Note that we clustered M- and E-DN in the left brain hemisphere to indicate the expression of the Clk4.1M-gal4 driver line. In the case of the M-LN, only the s-LNv are bonafide M-oscillators, but by using the Pdf-gal4 driver, we rescued PER also in the l-LNv. In the case of E-LN, we rescued PER in the sNPF-positive (E1) and ITP-positive (E2) neurons (using the PDF-gal80 Mai179-gal4 driver). The Cryptochrome (CRY)-negative neurons (E3) are not included.