TABLE 1.

Primers and PCR conditions for 16S and ITS amplicons.

Target	Cycle	Initial	Disassociate	Anneal	Extension	Finish
ITS1F–ITS2	35	95°C for 7 min	94°C for 30 s	55°C for 45 s	72°C for 60 s	72°C for 7 min
16S: V3–V4	29	95°C for 7 min	94°C for 30 s	50°C for 60 s	72°C for 60 s	72°C for 7 min