Figure 3. (A, B) Upregulation of COX-2 in colitis-associated cancer as determined by (A) reverse transcription-PCR for COX-2 mRNA or (B) Western blot analysis for COX-2 protein in colonic tissues.

Expression of COX-2 as well as its mRNA transcript was significantly increased in the colon of azoxymethane (AOM) plus dextran sulfate sodium (DSS) treated mice compared to the mice in the vehicle group. (C) Comparison of the relative expression of COX-2 and COX-1 and their mRNA transcripts between the vehicle group and the AOM plus DSS group. (D) Immunohistochemical staining of COX-2. Aberrantly increased expression of COX-2 was observed in infiltrated inflammatory cells and some epithelial cells surrounding the tumor (× 100). GAPDH, glyceraldegyde-3-phosphate dehydrogenase.