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. 2020 Mar 30;25(1):27–37. doi: 10.15430/JCP.2020.25.1.27

Figure 4. Effects of the COX-2 inhibitor celecoxib on azoxymethane (AOM) plus dextran sulfate sodium (DSS)-induced colon carcinogenesis and pro-inflammatory signaling.

Figure 4

Mice were treated with a single intraperitoneal injection of AOM and/or subsequent administration of 2% DSS in drinking water for 7 weeks. Control animals were given the vehicle alone. Celecoxib (0.1 mmole/kg or 0.25 mg/kg) was given orally by gastric intubation for 14 weeks. (A, B) Tumor incidences and tumor multiplicities. (C) The expression of COX-2 and inducible nitric oxide synthase (iNOS) in the colon of mice measured by Western blot analysis elevated. (D) DNA binding activity of NF-κB determined by the gel shift assay as described in Materials and Methods. (E) Inhibitory effects of celecoxib administration on IκBα degradation induced by AOM and DSS. The expression of IκBα was measured by Western blot analysis.