Effect of BRS3 manipulation in MC4R-expressing neurons on glucose homeostasis. (A) Insulin tolerance test (ITT) at 19 weeks of age (B) glucose area under the curve (AUC), (C) fed glucose, (D) fed insulin, and (E) body weight (BW) at the time of the test. (F) Glucose tolerance test (GTT) at 18 weeks of age (G) glucose area under the curve (AUC), (H) pre-test fasting glucose, (I) insulin at baseline (0 min) and 15 min after glucose dosing, and (J) body weight (BW) at the time of the test. Data are mean ± SEM, N = 8–13/group. P values were determined by t-test for the effect of Mc4r-Cre within each Brs3 genotype.