Role of BRS3 in SIM1-expressing neurons on body weight and energy intake. Mice were singly housed and fed a high fat diet (HFD) starting at 8 weeks of age. (A) Body weight, (B) fat mass, (C) lean mass, (D) food intake, and (E) metabolic efficiency over 8–16 weeks of age. (F) P values by t-test for the littermate comparisons in the indicated panels and times. Data are mean ± SEM, N = 8–13/group.