Fig. S1.

Exogenous spermine upregulates Nrf2 expression. (a) Western blot was used to detect Nrf2 expression in T1D rats, the representative band and quantification of Nrf2 protein expression was shown, (b) Western blot was used to detect Nrf2 and SOD2 expression in vitro, the representative band and quantification of Nrf2 and SOD2 protein expression was shown, (c) Western blot was used to detect the transfection efficiency of Nrf2-siRNA, the representative band and quantification of Nrf2 expression was shown, (d) SOD2 content and (e) CAT content were detected in cell lysates. *, P < 0.05 versus Control group; #, P < 0.05 versus T1D group or HG group.