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. 2020 Mar 21;32:101514. doi: 10.1016/j.redox.2020.101514

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 5 — Exogenous spermine protects cardiomyocytes by attenuating ROS level, stabilizing the redox state of cells, and up-regulating the expression of CaSR. By using NAC (ROS scavenger), we detected (a) The ROS level, (b) The expression of ER stress related proteins, (c) MDM2, MDM4 and p53 proteins and (d) CaSR synthesis related proteins. To further proved the effect of p53 on regulation of CaSR, OAZ1 overexpression was used. (e) Western blot was used to detect the overexpression efficiency of OAZ1, and (f) Immunoprecipitation was used to detect the formation of MDM4-MDM2 and MDM4-p53 complex. ***, P < 0.001 versus Control group; #, P < 0.05 versus HG group; ###, P < 0.001 versus HG group.