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. 2020 Mar 21;32:101514. doi: 10.1016/j.redox.2020.101514

Fig. 6.

Fig. 6

p53 inhibits PPARα expression by activating MuRF1, which can affect the process of CaSR synthesis. (a) The effect of OAZ1 overexpression on the cytomembrane CaSR expression. MuRF1-siRNA was used to transfect cardiomyocytes to verify its involvement in regulating cytomembrane CaSR synthesis, and (b) Western blot was used to detect the transfect efficiency of MuRF1-siRNA, the representative band and quantification of MuRF1 expression was shown, (c) The representative bands of MuRF1, PPARα and CaSR expression change, (d) Quantification of MuRF1, PPAR α and CaSR expression statistics. (e) Western blot was used to detect the overexpression efficiency of MuRF1, the representative band and quantification of MuRF1 expression was shown, (f) The representative bands of MuRF1 and CaSR expression change, and (g) Quantification of MuRF1 and CaSR expression statistics. ***, P < 0.001 versus Control group or Control + Scrambled-siRNA group or Control + Scrambled-Plasmid group; ###, P < 0.001 versus HG + Scrambled-siRNA group or HG + Scrambled-Plasmid group; &&&, P < 0.001 versus HG + MuRF1-Plasmid group.