Skip to main content
. 2020 Feb 26;23(3):100867. doi: 10.1016/j.isci.2020.100867

Figure 5.

Figure 5

Direct Induction of a Neutralizing Chimeric Antibody (ncAb) as Output Response in α-HBs SNR Cells

(A) α-HBs SNR cell design for induction of α-preS1 (#33) ncAb and gene construct of chimeric antibody with heavy chain and light chain linked by T2A sequence.

(B) Experimental design for induction of ncAb in α-HBs SNR cells and quantification of induced ncAb derived from α-HBs SNR cells. ncAb was induced from α-HBs SNR cells by co-culture with Tet-ON-SHBs cells in the presence of 5μg/mL doxycycline (Dox). After 48 h, ncAb in the cell supernatant were quantified by AlphaScreen assay. Control cells indicate Jurkat T cells harboring only ncAb gene without α-HBs SNR. The data shown represent the mean ± standard deviation (SD) of three independent experiments. ***p: < 0.001.

(C) Secreted ncAb suppresses HBV-NL infection in PXB cells. Schematic diagram of HBV-NL infection experiment timeline. PXB cells were infected with HBV-NL in the presence or absence of secreted ncAb derived from α-HBs SNR cells. Infectivity was determined by measuring luciferase activity 11 days after infection. Control cells indicate the cell supernatant of Jurkat T cells harboring only ncAb gene without α-HBs SNR co-cultured with Dox-treated Tet-ON-SHBs cells. Values obtained from supernatant of control cells were set as 100%, and the presented data represent the mean ± SD of three independent experiments. **p: < 0.01.