Figure 2.

Quantification of markers of oxidative stress and inflammation in muscles of mdx mice after AdipoRon treatment. For each immunolabelling of Figure 1A–1E, the percentage of DAB deposit areas was calculated within muscle sections. The subsequent ratios were presented as relative expression compared with WT values. ELISA assays were used to quantify HNE (F), TNFα (G), and IL‐1β (H) in the three groups of mice. Results are represented as protein concentrations (pg/mL, ng/mL, or μg/mL). (I, J) mRNA and protein levels of IL‐10, an anti‐inflammatory cytokine. mRNA levels were normalized to cyclophilin, and the subsequent ratios were presented as relative expression compared with WT values. Protein levels were quantified by ELISA and expressed as pg/mL. Data are means ± standard deviation; n = 6 mice per group for all experiments. Statistical analysis was performed using one‐way analysis of variance followed by Tukey's test. ^** P < 0.01, ^*** P < 0.001, ^**** P < 0.0001 vs. WT mice. ^#### P < 0.0001 vs. mdx mice.