Figure 6.

Effects of AdipoRon treatment on muscle injury and function in mdx mice. Muscle injury was assessed, in non‐exercised mice, by plasma activity of CK (A) and LDH (B) expressed as IU/L. Data are means ± standard deviation; n = 6 mice per group for both experiments. Functional in vivo studies were carried out in mice from the three groups. (C) Mice were subjected to a wire test where they were suspended by their limbs and the time until they completely released the wire and fell was recorded (seconds). Mice were lowered on a grid connected to a sensor to measure the muscle force of their forelimbs (D) or of both their forelimb and hindlimb (E); data were then expressed in gram force relative to body weight (gf/gBW). (F) Mice were submitted to a downhill treadmill exercise for 10 min during three consecutive days. On the third session, the covered distance (metres) was measured for each mouse, 100 m being the maximal distance. Data are means ± standard deviation; n = 9 mice per group for all in vivo tests. Statistical analysis was performed using one‐way analysis of variance followed by Tukey's test. ^* P < 0.05, ^**** P < 0.0001 vs. WT mice. ^## P < 0.01, ^#### P < 0.0001 vs. mdx mice.