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. 2020 Mar 11;21(2):e33. doi: 10.4142/jvs.2020.21.e33

Table 1. List of primers used to create AnxA2-WT and AnxA2 deletion mutants.

Primer name Sequence (5′-3′) Application
AnxA2-WT-F CGCGGATCCGCCACCATGTCTACCGTTCATGAAATTC Construct recombinant plasmids of pLVX-IRES-Puro
AnxA2-WT-R CCGGAATTCTCACTTGTCATCGTCGTCCTTGTAATCGTCATCCCCACCACACAGG
AnxA2-ΔI-F CGCGGATCCGCCACCATGCGGGATGCTCTGAACATTG Construct recombinant plasmids of pcDNA3.1(+)
AnxA2-ΔI-R CCGGAATTCTCACTTGTCATCGTCGTCCTTGTAATCGTCATCCCCACCACACAGG
AnxA2-ΔII-R AGCAGGTGTTTTCAACTCAGCATCAAAGTTAGTG
AnxA2-ΔII-F AACTTTGATGCTGAGTTGAAAACACCTGCTCAGT
AnxA2-ΔIII-F AAAACACCTGCTCAGGCAAAGGGTCGGAGAGCAG
AnxA2-ΔIII-R TCTCCGACCCTTTGCCTGAGCAGGTGTTTTCAAT
AnxA2-ΔIV-F TATGAACTGATTGACGTTCAGTGCATTCAGAACA
AnxA2-ΔIV-R CTGAATGCACTGAACGTCAATCAGTTCATAATCA
AnxA2-ΔV-F CGCGGATCCGCCACCATGTCTACCGTTCATGAAATTC
AnxA2-ΔV-R CCGGAATTCTCACTTGTCATCGTCGTCCTTGTAATCGTCATCCCCACCACACAGGGGCTTGTTCTGAATG

Bold letters indicate the restriction endonuclease enzyme cutting sites; Underlined letters indicate the Flag-Tag sequence.

AnxA2, annexin A2; WT, wild type; F, forward; R, reverse.