Table 1. List of primers used to create AnxA2-WT and AnxA2 deletion mutants.
| Primer name | Sequence (5′-3′) | Application |
|---|---|---|
| AnxA2-WT-F | CGCGGATCCGCCACCATGTCTACCGTTCATGAAATTC | Construct recombinant plasmids of pLVX-IRES-Puro |
| AnxA2-WT-R | CCGGAATTCTCACTTGTCATCGTCGTCCTTGTAATCGTCATCCCCACCACACAGG | |
| AnxA2-ΔI-F | CGCGGATCCGCCACCATGCGGGATGCTCTGAACATTG | Construct recombinant plasmids of pcDNA3.1(+) |
| AnxA2-ΔI-R | CCGGAATTCTCACTTGTCATCGTCGTCCTTGTAATCGTCATCCCCACCACACAGG | |
| AnxA2-ΔII-R | AGCAGGTGTTTTCAACTCAGCATCAAAGTTAGTG | |
| AnxA2-ΔII-F | AACTTTGATGCTGAGTTGAAAACACCTGCTCAGT | |
| AnxA2-ΔIII-F | AAAACACCTGCTCAGGCAAAGGGTCGGAGAGCAG | |
| AnxA2-ΔIII-R | TCTCCGACCCTTTGCCTGAGCAGGTGTTTTCAAT | |
| AnxA2-ΔIV-F | TATGAACTGATTGACGTTCAGTGCATTCAGAACA | |
| AnxA2-ΔIV-R | CTGAATGCACTGAACGTCAATCAGTTCATAATCA | |
| AnxA2-ΔV-F | CGCGGATCCGCCACCATGTCTACCGTTCATGAAATTC | |
| AnxA2-ΔV-R | CCGGAATTCTCACTTGTCATCGTCGTCCTTGTAATCGTCATCCCCACCACACAGGGGCTTGTTCTGAATG |
Bold letters indicate the restriction endonuclease enzyme cutting sites; Underlined letters indicate the Flag-Tag sequence.
AnxA2, annexin A2; WT, wild type; F, forward; R, reverse.