FIG 8.

ST induces changes epithelial gene expression. STh (100 ng) was added to three confluent T84 epithelial monolayers for 3 h before RNA isolation. Another set of three monolaters were left untreated to serve as the control. RNA was isolated from all six monolayers and reverse transcribed into cDNA using iScript. qPCR was carried out using predesigned gene-specific primers from IDT, PrimeTime gene expression master mix, and approximately 10 ng of cDNA input from both ST-treated and untreated samples on a CFX Connect. HPRT1 and ACTB were used as housekeeping genes, and the y axis represents the changes in transcript level of genes from T84 cells treated with ST compared to that in untreated cells.