FIG 3.

Preincubation of endothelial cells with Tp0751 enhances adhesin attachment. Binding assay evaluating attachment of FITC-labeled Tp0751 to endothelial cells following HUVEC preincubation with 30, 40, or 50 μM unlabeled Tp0751 (E115–P237) or control Tp0327 (I23–S172). Results are presented as means plus SEM from duplicate wells in two independent experiments and expressed as percent binding of the FITC-Tp0751-only control (no unlabeled protein added; set at 100%). Statistical analyses were performed by one-way ANOVA comparing binding of FITC-Tp0751 to endothelial monolayers preincubated with Tp0751 or negative-control Tp0327 (P = 0.004).