Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 1;5(2):e00195-20. doi: 10.1128/mSphere.00195-20

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Lithgow et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

PMC Copyright notice

FIG 5 — Recombinant Tp0751 interacts with endogenously expressed LamR in brain endothelial cells (bECs). Live hCMEC/d3 monolayers were left untreated (bEC lysate) or coincubated with exogenous recombinant Tp0751 (bEC Lysate + Tp0751). (A) Lysates were incubated with magnetic beads in the absence of antibodies (Lysate + Bead Controls) to control for nonspecific interactions between LamR and the beads. LamR was immunoprecipitated (IP) from lysates using mouse anti-LamR antibody (α-LamR) covalently coupled to magnetic beads, and coimmunoprecipitation of Tp0751 was evaluated by immunoblotting (IB) against Tp0751 (aa 115–237) and LamR. Representative results from three independent experiments are shown. (B) Quantitative immunoblot of LamR (red) and internal control protein GAPDH (green) from untreated or Tp0751-treated bEC lysates (C) LamR band intensity normalized to GAPDH band intensity from untreated or Tp0751-treated bEC lysates. Results representative of two independent experiments.