FIG 6.

Reciprocal binding between recombinant Tp0751 and LamR. (A) Binding of recombinant Tp0751 (V99–P237) or control treponemal protein Tp0327 (I23–S172) to immobilized LamR. (B) Binding of recombinant LamR to immobilized Tp0751 (V99–P237) or Tp0327 (I23–S172). Results are presented as mean optical density at 600 nm plus SEM from duplicate wells in three independent experiments for LamR-immobilized binding assays and from duplicate wells in two independent experiments for Tp0751-immobilized binding assays. Statistical analyses were performed by Student’s two-tailed t test comparing binding of Tp0751 and Tp0327 to immobilized LamR (P = 0.0157) and LamR binding to immobilized Tp0751 versus immobilized Tp0327 (P = 0.0447).